NEWS
How Promega NanoLuc® Technology Works
NanoLuc® Luciferase (NanoLuciferase) is a small (19 kDa), engineered enzyme derived from the deep-sea shrimp Oplophorus gracilirostris. Unlike traditional luciferases, it utilizes a novel substrate (furimazine) to produce a bright, glow-type luminescence with exceptional stability. This unique combination enables a range of assay formats—from simple reporter gene detection to sophisticated live-cell and multiplexed systems.
All NanoLuc®-based assays share three core benefits:
- Ultra-bright signal – 100–1000x brighter than firefly or Renilla luciferase
- Minimal interference – Small size allows fusion protein design without disrupting native biology
- Wide dynamic range – Reliable detection across a broad range of expression levels
Perfect for gene expression analysis, protein-protein interaction analysis, and high-throughput screening workflows!
Promega NanoLuc® Assay Systems at a Glance
- NanoLuc® Luciferase System: Best for simple reporter gene assays, promoter analysis, signaling pathways
- NanoLuc® Live Cell System: Best for real-time monitoring of gene expression in living cells
- Endurazine™ and Vivazine® substrates: Best for long-term kinetic studies; chronic treatment experiments
- Nano-Glo® Dual-Luciferase® Reporter Assay System: Best for multiplexed assays; normalize expression with internal controls
Why Choose NanoLuc® Assay Systems?
NanoLuc® technology offers a unified platform that scales from simple endpoint detection to sophisticated live-cell and multiplexed formats. Whether you are screening compound libraries, studying dynamic cellular responses, or building complex reporter systems, there is a NanoLuc® solution designed to meet your needs.
- Scalable – Compatible with 96-, 384-, and 1536-well formats for HTS applications
- Flexible – Lytic, live-cell, and long-term kinetic options available
- Reliable – Consistent performance across multiple cell types and experimental conditions
As a Promega authorized distributor in Hong Kong, we provide full technical support and product expertise to help you select the right assay system for your research.
For more information on Promega Bioluminescence assay systems, or help selecting the right system for your research, please contact us!
NanoLuc® 檢測系統選擇指南：為你的研究挑選最合適的方案
NanoLuc® 冷光酵素技術以其卓越的亮度、小巧的分子結構與穩定的訊號，徹底改變了生物冷光檢測的應用模式。隨著多種檢測系統的推出——每種系統皆針對特定實驗需求設計——如何選擇合適的方案可能令人感到困惑。本指南將為你介紹各項 NanoLuc® 檢測系統，協助你根據實驗需求找到最匹配的工具。
NanoLuc® 技術原理
NanoLuc® 冷光酵素是一種小分子（19 kDa）的工程化酵素，源自深海蝦 Oplophorus gracilirostris。與傳統冷光酵素不同，它採用新型受質（furimazine），能產生明亮且穩定的冷光訊號。這項獨特的組合使其能夠支援多種檢測形式——從簡單的報導基因檢測，到複雜的活細胞與多重檢測系統。
所有 NanoLuc® 檢測系統皆具備三大核心優勢：
- 超高亮度訊號 – 亮度為螢火蟲或海腎冷光酵素的 100–1000 倍
- 低干擾性 – 分子小，適合設計融合蛋白，不影響原生生物功能
- 寬廣動態範圍 – 在不同表現量下皆能穩定偵測
非常適合基因表達研究、蛋白質交互作用分析及高通量篩選流程！
NanoLuc® 檢測系統一覽
- NanoLuc® 冷光酵素系統：適用於簡易報導基因檢測、啟動子分析、訊號傳遞路徑研究
- NanoLuc® 活細胞檢測系統：適用於即時監測活細胞中的基因表現
- Endurazine™ 與 Vivazine® 受質：適用於長期動力學研究、慢性給藥實驗
- Nano-Glo® 雙冷光酵素報導基因檢測系統：適用於多重檢測、以內部對照組正規化表現量
為甚麼選擇 NanoLuc® 檢測系統？
NanoLuc® 技術提供了一個統一的平台，可從簡單的終點檢測擴展至複雜的活細胞與多重檢測形式。無論你正在篩選化合物庫、研究動態細胞反應，或是建立複雜的報導基因系統，都能找到適合你需求的 NanoLuc® 解決方案。
- 可擴展性 – 支援 96、384 及 1536 孔盤格式，適用於高通量篩選應用
- 靈活性 – 提供裂解型、活細胞型與長期動力學型等多種選擇
- 穩定性 – 在多種細胞株與實驗條件下皆能提供一致的表現
作為Promega 香港授權經銷商，我們提供全面的技術支援與產品專業知識，協助你為研究挑選最合適的檢測系統。
如想了解更多關於Promega 生物冷光檢測系統的資訊，或協助挑選適合你研究的系統，歡迎與我們聯絡！
